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Alternatively, the inventory tradition organisms can be grown in an appropriate liquid medium (i.e., SoybeanCasein Digest Broth or Sabouraud Dextrose Broth) and the cells harvested by centrifugation, then washed and resuspended in sterile saline TS to get a microbial count of about one × 108 cfu for each mL. [Take noteThe estimate of i